A 69-year-old Drouin man died after crashing his Harley trike in Longwarry on Saturday night.
Police said the fatality occurred in Mackey St at about 9.45pm.
They believe the trike was travelling east when the rider lost control. Despite emergency services quickly arriving on the scene, the rider died.
Police said they were still investigating the exact circumstances of the incident.
Anyone who witnessed the crash or has footage is urged to contact Crime Stoppers on 1800 333 000 or visit crimestoppersvic.com.au
The Drouin man is the seventh life lost on Baw Baw roads this year.